Effect of EGTA on serotonin transport into blood platelets.
نویسندگان
چکیده
منابع مشابه
Spectrophotofluorometry of serotonin in blood platelets.
A rapid sensitive, and reproducible assay for determination of platelet serotonin is reported. Serotonin in extracted into an ascorbic acid solution by freezing and thawing and sonication. Ascorbic acid stabilizes the serotonin in the extract, and added ethanol enhances the final fluorescence of serotonin, which is measured in a concentrated hydrochloric acid medium (activation at 295 nm and em...
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15 صفحه اولThe electron transport chain of serotonin-dense granules of platelets.
An electron transport chain has been found and partially characterized in highly purified membranes of serotonin granules isolated from porcine platelets. In these membranes NADH, but not NADPH, is oxidized when ferricyanide or catalytic amounts of cytochrome c were present. The reduced minus oxidized spectrum of these serotonin granule membranes shows a characteristic cytochrome b spectrum wit...
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Repeated thrombin treatment of washed platelets prepared from rabbits can decrease the serotonin content of the platelets by about 80%. When these platelets are deaggregated they reaccumulate serotonin but their storage capacity for serotonin is reduced by about 60%. If thrombin-pretreated platelets are allowed to equilibrate with a high concentration of serotonin (123 mu M), they release a sma...
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ژورنال
عنوان ژورنال: Japanese Journal of Pharmacology
سال: 1993
ISSN: 0021-5198
DOI: 10.1016/s0021-5198(19)51499-8